Cory Monteith, Kevin McHale, Naya Rivera and Jenna Ushkowitz are also scheduled to appear alongside Michele and Criss for the panel, reports TV Line.
Glee co-creator Falchuk expressed excitement to appear at Comic-Com, saying: "The fans at Comic-Con were among the first to really get behind our show, and we've had such a great time returning there every summer.
"This was the first year our cast has not gone out on tour, so Ryan and I thought we'd let them have some well-deserved time off. But everyone wanted to be there, so we couldn't be happier that Glee is returning to the convention where we feel it all started."
Glee's Comic-Con panel will take place on Saturday, July 14 from 6-7pm.