However, Apatow told E! News that he believes she will be a part of the film.
"She will be back!" he said.
Applegate has said that she would like to return if possible, describing Anchorman as the "best job" she's ever had and saying: "Of course I would love to be there. They know that. The world knows that. Why would I not want to?"
Meanwhile, Apatow revealed that the team behind the Anchorman sequel are hoping for "a lot" of celebrity cameos, saying: "I just don't know who they're specifically thinking about."